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Exactly about Gene Transfer and Genetic Recombination in Bacteria

The following points highlight the 3 modes of gene transfer and hereditary recombination in bacteria. The modes are: 1. Transformation 2. Transduction 3. Bacterial Conjugation.

Mode number 1. Transformation:

Historically, the breakthrough of change in germs preceded one other two modes of gene transfer. The experiments carried out by Frederick Griffith in 1928 suggested for the very first time that a gene-controlled character, viz. Development of capsule in pneumococci, could possibly be utilized in a variety that is non­-capsulated of bacteria. The transformation experiments with pneumococci eventually resulted in a discovery that is equally significant genes are constructed with DNA.

Within these experiments, Griffith utilized two strains of pneumococci (Streptococcus pneumoniae): one with a polysaccharide capsule creating ‘smooth’ colonies (S-type) on agar plates that has been pathogenic. One other strain ended up being without capsule creating ‘rough’ colonies (R-type) and had been non-pathogenic.

As soon as the living that is capsulated (S-bacteria) had been inserted into experimental pets, like laboratory mice, a substantial percentage for the mice passed away of pneumonia and live S-bacteria could be isolated through the autopsied pets.

As soon as the non-capsulated living pneumococci (R-bacteria) were similarly inserted into mice, they stayed unaffected and healthier. Additionally, whenever S-pneumococci or R-pneumococci had been killed by temperature and injected separately into experimental mice, the animals failed to show any condition symptom and remained healthy. But a unanticipated outcome had been experienced whenever a combination of residing R-pneumococci and heat-killed S-pneumococci had been inserted. Weiterlesen